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. 2005 Oct 31;102(45):16385–16390. doi: 10.1073/pnas.0506081102

Table 2. Mating experiments to check for protein transfer into recipient cells.

Transfer elements in strain 1
R388 mutation in strain 2
Experiment Plasmids oriT trwA trwB trwC T4SS R388 T4SS pKM101 oriT-mob CloDF13 Transfer frequency
   1. trwC control pKM101Δmob + pET29c + trwC (pSU1445) <10–7
   2. trwB control pKM101Δmob + pET29c + trwB (pSU1443) <10–7
   3. trwA control pKM101Δmob + pET29c + trwA (pSU4028) 8 × 10—6
   4. TrwC transfer with DNA transfer pKM101Δmob + pET29:trwABC + pSU1371 + + + + + trwC (pSU1445) 8 × 10—3
   5. TrwB transfer with DNA transfer pKM101Δmob + pET29:trwABC + pSU1371 + + + + + trwB (pSU1443) 8 × 10—7
   6. TrwA transfer with DNA transfer pKM101Δmob + pET29:trwABC + pBBR::oriT + + + + + trwA (pSU4028) 2 × 10—6
   7. TrwC transfer w/o DNA transfer pKM101Δmob + pET29:trwABC + + + + trwC (pSU1445) 5 × 10—5
   8. TrwB transfer w/o DNA transfer pKM101Δmob + pET29:trwABC + + + + trwB (pSU1443) 2 × 10—7
   9. TrwA transfer w/o DNA transfer pKM101Δmob + pET29:trwABC + + + + trwA (pSU4028) 5 × 10—6
10. TrwC transfer w/CloDF DNA transfer pKM101Δmob + pET29:trwABC + pSU4814 + + + + + trwC (pSU1445) 5 × 10—5
11. Negative control for CloDF13 pKM101Δmob + pET29c + pSU4814 + + trwC (pSU1445) <10—7
12. TrwC transfer w/o coupling protein pKM101Δmob + pET29:trwAC + + + trwC (pSU1445) 2 × 10—7
13. TrwC transfer with entry exclusion pSU711ΔoriT::aac3 + + + + trwC (pSU1445) <10—7
14. TrwB transfer with entry exclusion pSU711ΔoriT::aac3 + + + + trwB (pSU1443) <10—7

Triparental matings were performed as explained in Methods. The first column describes the aim of each experiment. To facilitate interpretation, the elements of the conjugative machinery present in strain 1 are indicated. Frequencies represent number of transconjugants per strain 2. Figures are the mean of two to six experiments.