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| Goal of experiment | Helper plasmid | TrwC protein | Percent blue |
|---|---|---|---|
| TrwC-mediated recombination | pET3::trwAC | Wild-type | 100.0 |
| Strand-transfer mutant | pET3::trwACmut | Y18FY26F | 0.7 |
| Negative control | pSU1547 | None | 0.7 |
Transformation of helper plasmid into DH5α (pRec2oriT-Cm). Blue colonies screened after 40 generations of growth. Data are the mean of three independent assays.
