Table 4. oriT-specific recombination in recipient cells.
Goal of experiment | Plasmids in donor | Plasmid features | Percent blue |
---|---|---|---|
Recombination in recipient (TrwC-DNA transfer) | pSU711ΔoriT + pSU1371 | R388 w/o oriT + vector::oriT | 10.0 |
Negative control (MobC–DNA transfer) | pClomob::oriT + pSU4058 | CloDF13 w/R388 oriT + vector::T4SS (R388) | 0.1 |
Conjugation from D1210 into DH5α (pRec2oriT-Ap). Direct count of blue/white transconjugants. Data are the mean of three independent assays.