Table 1. Quantification of APC mutations in plasma.
| Patient no. | Sex/age, yr | Site | Dukes' stage (tumor node metastasis stage) | Diameter of lesion, cm | Mutation identified in primary tumor (codon) | Fragments per ml of plasma | No. of fragments analyzed | Percentage of mutant fragments, % |
|---|---|---|---|---|---|---|---|---|
| 1 | M/50 | Ascending colon | Adenoma | 3.0 | C4348T (1450) | 2,600 | 2,350 | 0.002 |
| 2 | M/67 | Descending colon | Adenoma | 2.5 | C4285T (1429) | 5,080 | 5,080 | 0.001 |
| 3 | M/54 | Rectum | Adenoma | 4.0 | G3856T (1286) | 4,150 | 4,150 | 0.002 |
| 4 | F/82 | Rectum | Adenoma | 3.0 | 4147–4148insA (1383) | 1,350 | 1,350 | 0.001 |
| 5 | F/65 | Rectum | Adenoma | 1.0 | C4067G (1356) | 4,260 | 4,260 | 0.001 |
| 6 | F/71 | Ascending colon | Adenoma | 4.0 | G3856T (1286) | 4,150 | 4,150 | 0.001 |
| 7 | M/68 | Cecum | Adenoma | 6.5 | C4285T (1429) | 4,760 | 4,760 | 0.003 |
| 8 | M/93 | Ascending colon | Adenoma | 0.8 | A4345T (1449) | 4,320 | 4,320 | 0.001 |
| 9 | F/78 | Ascending colon | Adenoma | 3.0 | C4216T (1406) | 28,570 | 28,570 | 0.001 |
| 10 | F/59 | Sigmoid colon | Adenoma | 5.0 | 4661–4662insA (1554) | 2,160 | 2,160 | 0.002 |
| 11 | F/73 | Ascending colon | Adenoma | 5.0 | C4348T (1450) | 8,000 | 8,000 | 0.02 |
| Median/mean | 4,300/6,300 | 0.02* | ||||||
| Mutant plasma samples per samples analyzed | 1/11 (9) | |||||||
| 12 | F/81 | Sigmoid colon | A (T2N0M0) | 4.0 | G4189T (1397) | 7,900 | 12,000 | 0.01 |
| 13 | F/75 | Sigmoid colon | A (T2N0M0) | 2.5 | 3927–3931del AAAGA (1309) | 2,160 | 2,160 | 0.001 |
| 14 | M/60 | Sigmoid colon | A (T2N0M0) | 3.0 | 3927–3931del AAAGA (1309) | 4,600 | 6,900 | 0.04 |
| 15 | M/79 | Right colic flexure | A (T2N0M0) | 3.0 | 4470delT (1490) | 4,600 | 3,696 | 0.03 |
| 16 | M/70 | Ileocecal | A (T2N0M0) | 2.5 | 4481delA (1494) | 6,200 | 3,105 | 0.07 |
| 17 | F/68 | Ascending colon | A (T2N0M0) | 3.5 | C4348T (1450) | 2,170 | 2,170 | 0.001 |
| 18 | F/66 | Sigmoid colon | A (T1N0M0) | 2.5 | 3927–3931del AAAGA (1309) | 1,920 | 1,920 | 0.001 |
| 19 | M/68 | Rectum | A (T2N0M0) | 5.5 | C3907T (1303) | 2,300 | 1,170 | 0.12 |
| Median/mean | 3,500/4,000 | 0.04/0.04* | ||||||
| Mutant plasma samples per samples analyzed | 5/8 (63) | |||||||
| 20 | F/65 | Cecum | B (T3N0M0) | 3.5 | G4396T (1466) | 5,300 | 5,300 | 0.002 |
| 21 | M/71 | Sigmoid colon | B (T3N0M0) | 3.0 | C4348T (1450) | 2,100 | 1,863 | 0.19 |
| 22 | M/37 | Descending colon | B (T4N0M0) | 10.0 | C4330T (1444) | 5,400 | 4,887 | 1.28 |
| 23 | M/64 | Sigmoid colon | B (T3N0M0) | 6.5 | C4099T (1367) | 3,810 | 3,810 | 0.001 |
| 24 | M/72 | Sigmoid colon | B (T3N0M0) | 3.0 | C4012T (1338) | 4,800 | 4,800 | 0.03 |
| 25 | F/82 | Hepatic flexure | B (T3N0M0) | 4.0 | C4099T (1367) | 3,840 | 3,840 | 1.46 |
| 26 | M/83 | Ascending colon | B (T3N0M0) | 6.0 | 4470delT (1490) | 1,600 | 1,404 | 1.75 |
| 27 | M/61 | Sigmoid colon | B (T3N0M0) | 4.0 | 4260–4261delCA (1420) | 4,200 | 4,200 | 0.001 |
| Median/mean | 4,000/3,900 | 1.28/0.94* | ||||||
| Mutant plasma samples per samples analyzed | 5/8 (63) | |||||||
| 28 | F/83 | Ascending colon | D (T3N2M1) | 5.0 | 4661–4662insA (1554) | 230,000 | 24,857 | 5.6 |
| 29 | M/55 | Sigmoid colon | D (T3N0M1) | 3.0 | G3925T (1309) | 69,600 | 1,636 | 27.4 |
| 30 | F/33 | Descending colon | D (T4N1M1) | 5.0 | C4067A (1356) | 18,000 | 491 | 10.5 |
| 31 | M/64 | Sigmoid colon | D (T4N2M1) | 6.0 | T4161A (1387) | 26,000 | 975 | 1.9 |
| 32 | M/56 | Rectum | D (T3N2M1) | 3.0 | 4468–4469delCA (1490) | 103,200 | 1,187 | 18.9 |
| 33 | F/60 | Rectum | D (T3N2M1) | 4.0 | 4059–4060insT (1354) | 8,400 | 850 | 2.0 |
| Median/mean | 47,800/75,900 | 8.05/11.05* | ||||||
| Mutant plasma samples per samples analyzed | 6/6 (100) | |||||||
*
Calculated only for samples in which the percentage of mutant fragments was significantly higher than in control samples (i.e., >0.003%; printed in boldface). M, male; F, female