Table 1. Listeria-induced actin assembly.
| Cell line | Infection rate, % | Bacteria with actin tail, % | Tail length, μm | Total Listeria associated F-actin |
|---|---|---|---|---|
| WT | 45 ± 1.4 | 42 ± 2.9 | 9.7 ± 0.3 | 407.4 |
| pMSCV | 46 ± 6.8 | 48.3 ± 4.2 | 10.7 ± 0.4 | 516.8 |
| pMh | 59 ± 3 | 58 ± 6 | 7.8 ± 0.3 | 452.4 |
| cl.4-3 | 59 ± 6.8 | 22.5 ± 6.5 | 5.9 ± 0.2 | 132.75 |
| cl.7-4 | 27.6 ± 2.8 | 9.8 ± 1 | 4.4 ± 0.2 | 43.1 |
| cl.7-14 | 42.5 ± 3.5 | 11 ± 0.6 | 4.1 ± 0.3 | 45.1 |
Quantification of Listeria infection rates (means ± SD of three experiments, 100 cells counted per experiment), percentage of bacteria with actin tails (100–130 bacteria counted per experiment), and lengths of the actin comet tails (110–190 bacteria counted per experiment). Total F-actin associated with Listeria was estimated in arbitrary units in each cell line by multiplying the average number of bacteria with an actin comet tail and the average tail length. There was a 71% (cl.4-3) and a 90% (cl.7-4 and cl.7-14) reduction in F-actin associated with Listeria in comparison with the control cell lines.