TABLE 1.
Assay no.b | No. of observable foci with input IFUa
|
||||
---|---|---|---|---|---|
25.0 | 12.5 | 6.25 | 3.12 | 1.56 | |
1 | 26.0 | 11.0 | 5.0 | 3.0 | 1.0 |
22.0 | 13.0 | 7.0 | 1.0 | 3.0 | |
24.0 | 15.0 | 10.0 | 3.0 | 0.0 | |
25.0 | 15.0 | 6.0 | 4.0 | 2.0 | |
25.0 | 9.0 | 6.0 | 4.0 | 1.0 | |
Mean ± SEc | 24.4 ± 0.7 | 12.6 ± 1.2 | 6.8 ± 0.9 | 3.0 ± 0.5 | 1.4 ± 0.5 |
2 | 18.0 | 12.0 | 6.0 | 2.0 | 1.0 |
25.0 | 11.0 | 2.0 | 3.0 | 0.0 | |
25.0 | 15.0 | 6.0 | 3.0 | 4.0 | |
24.0 | 11.0 | 8.0 | 2.0 | 1.0 | |
28.0 | 13.0 | 4.0 | 3.0 | 4.0 | |
Mean ± SEc | 24.0 ± 1.6 | 12.4 ± 0.7 | 5.2 ± 1.0 | 2.6 ± 0.2 | 2.0 ± 0.8 |
Input IFU were calculated as 25 IFU per well, and four twofold serial dilutions were performed to obtain additional concentrations of 12.5, 6.25, 3.12, and 1.56 IFU per well.
Focal assays 1 and 2 consisted of five wells of each final concentration of input IFU.
Mean values (n = 5) ± standard error.