Fig. 3.

miR132 is transcribed from the stable intron of a cryptic noncoding RNA. (A) A diagram indicating the position of premiR132 and premiR212 and a unique rat noncoding transcript on chromosome 10 (UCSC Genome Center Rn3 assembly). A CpG island and mouse, rat, human, fugu, dog conservation track are indicated. All annotation is from the UCSC Genome Center. The red box in the RT-PCR track indicates a PCR product that interrogates premiR132. (B) Cortical neurons were treated with BDNF or vehicle for 60 min. RNA was reverse-transcribed and analyzed by real-time PCR by using primers interrogating the locations indicated in A. Error is SEM of four replicates.