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Splice-site recognition across the intron is more efficient than splice-site recognition across the exon. (A) Representative autoradiogram depicting the efficiency of splicing for substrates containing weak 5′ and 3′ splice sites that differ only in the length of their intron, as indicated above each panel. The diagrams (Right) represent pre-mRNA and spliced products. (B) Quantitation of the data in A.
