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Association of NCoR with PPRE-bound unliganded TRβ1 or PV is independent of troglitazone. In vitro-translated NCoR, PPARγ, TRβ1, or PV proteins (3.5 μl) in the presence or absence of 1 μMT3(A)or1 μM troglitazone (B) were incubated with 32P-labeled PPRE and analyzed by EMSA, as described in Materials and Methods. The broken arrow denotes the nonspecific binding.
