Fig. 6.
Recruitment of NCoR to PPRE-bound PPARγ and PV in the promoter of the LpL gene in the thyroid of TRβPV/PV mice by CHIP assay. Thyroid nuclear extracts from wild-type (W) or TRβPV/PV mice (P) were processed for CHIP assay, as described in Materials and Methods. Anti-PPARγ, anti-NCoR, anti-PV (polyclonal antibody, T1, or monoclonal antibody #302) (17) antibodies, and IgG (for negative controls) were used for immunoprecipitation. The precipitated DNA was amplified by PCR with primers specific for the LpL PPRE, and the products were analyzed. A representative example is shown here, but the experiments were repeated three times with similar results.