Table 2.

Melting temperatures of wild-type and mutant forms of human eRF1

No.b	Wild-type eRF1 and its mutants	Td (°C)	ΔTda (°C)
1	Wild-type	55.3	0
2	E55R	54.4	−0.9
3	E55D	55.8	+0.5
4	E55A	54.5	−0.8
5	Y125A	52.5	−2.8
6	Y125F	55.6	+0.3
7	N129A	53.7	−1.6
8	N129P	48.6	−6.7
9	F131A	52.6	−2.7
10	N129A + F131A	49.3	−6.0

^aΔT_d = T_d (mutant) − T_d (wt).

^bMutants numbers 7–10 were isolated and functionally tested earlier (12) and the calorimetric data are taken from (32) to show the sensitivity of the calorimetric method.