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. 2005 Nov 10;33(20):6461–6468. doi: 10.1093/nar/gki957

Figure 6.

Figure 6

DNA cleavage and TPRT activities of the Myb− mutant R2 protein. In each panel the WT and Myb− R2 proteins are compared at three protein concentrations. The proteins were equilibrated by RT activity in standard primer extension reactions (Materials and Methods). The diagrams compare the ability of the WT (black squares) and Myb− (gray diamonds) proteins to bind target DNA in EMSA assays (A), cleave the DNA on the bottom-strand (B), perform TPRT (C) and cleave the DNA on the top-strand (D). DNA-binding is reported as the fraction of DNA bound by protein as measured by EMSA gels. DNA cleavage is reported as the fraction of DNA cleaved divided by the fraction of bound DNA. TPRT is reported as the fraction of DNA that had undergone TPRT divided by the fraction of DNA that had been cleaved on the bottom-strand.