TABLE 1.
Proteins and peptides spanning the predicted HD of MSP1a
| Protein or peptidea | Amino acid positionb | Sequencec |
|---|---|---|
| Protein | ||
| MSP1a C-terminal region | End of repeats (68)-593 | Reference 5 |
| HD | 1-366 | Reference 5 |
| F1 | 68-180 | |
| F2 | 160-270 | |
| F3 | 250-366 | |
| Peptide | ||
| F2-1 | 151-180 | GLQGLKIGEGYATYLAQAFADSVVVAADVQ |
| F2-2 | 171-200 | DSVVVAADVQSSGACSASLDSAIANVETSW |
| F2-3 | 191-220 | SAIANVETSWSLHGGLVSKGFDRDTKVERG |
| F2-4 | 211-240 | FDRDTKVERGDLEAFVDFMFGGVSYNDGNA |
| F2-5 | 231-260 | GGVSYNDGNASAARSVLETLAGHVDALGIS |
| F3-1 | 250-279 | LAGHVDALGISYNQLDKLDADTLYSVVSFS |
| F3-2 | 270-299 | DTLYSVVSFSAGSAIDRGAVSDAADKFRVM |
| F3-3 | 290-319 | SDAADKFRVMMFGGAPAGQEKTAEPEHEAA |
| F3-4 | 310-339 | KTAEPEHEAATPSASSVPSTVHGKVVDAVD |
| F3-5 | 330-359 | VHGKVVDAVDRAKEAAKQAYAGVRKRYVAK |
| F3-6 | 350-366 | AGVRKRYVAKPSDTTTQ |
a
MSP1a was expressed as a fusion protein with MBP in E. coli (17). The HD and F1 to F3 proteins were expressed as His-tagged proteins in COS-7 cells.
b
Amino acid designation is based on the position in the VA strain, which contains two N-terminal repeats.
c
The peptides overlap by 10 or 11 aa, indicated by underlined sequences.