FIG. 2.

The elevation of intracellular cAMP activates MDDC. Cell surface expression of the indicated markers on untreated MDDC (dashed histograms) or on MDDC treated with the indicated stimulus (solid histograms) is shown. Day 4 MDDC were incubated with 1 μg of CT/ml, 1 mM d-cAMP, 2 mM butyric acid, or the indicated concentration of Forskolin for 20 h. The cells were then harvested and stained for four-color flow cytometry with phycoerythrin anti-CD80, fluorescein isothiocyanate anti-CD83, Cy-Chrome anti-CD86, or allophycocyanin anti-HLA-DR. The data shown are representative of five experiments performed.