FIG. 2.
Northern analysis of IFN-responsive gene expression in tissues recovered from uninfected and Salmonella-infected mice. Total RNA was isolated from Peyer's patches (PP), spleen, liver, and brain tissue (0.1 g) from BALB/c mice, either uninfected (U) or 6 days after oral infection with WT Salmonella. RNA samples (10 μg) were fractionated by formaldehyde-agarose gel electrophoresis, transferred to a nylon membrane, and then hybridized with 32P-labeled cDNA probes for mouse iNOS, Mx1, PKR, and ADAR1. The mouse ribosomal L3-2 protein probe was included as a control.