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P40 expression in M. agalactiae. Immunoblot analysis was carried with 10 μg of total antigen in each lane. Total antigen of all of the 27 M. agalactiae strains tested was separated by SDS-12% acrylamide gels, transferred onto a nitrocellulose membrane, and probed with antiserum to P40. The letter following each strain name indicates the serotype.
