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Electron micrographs showing strains ORN172(pJOR5) (A and B) and ORN172(pLPF100) (C) labeled with immunogold conjugated to S. enterica serovar Typhimurium LpfA antiserum. Bars, 0.2 μm. (D) Western blot analysis of fimbrial crude lysates from E. coli strains ORN172(pJOR5) (lane 1), 86-24 (lane 2), and CVD468 (lane 3) reacted with S. enterica serovar Typhimurium LpfA antiserum. LpfA is identified with an arrow, and the molecular mass markers (in kilodaltons) are indicated on the right.
