Phenotypic analysis of an auxin-resistant tRNAala anticodon mutant from Arabidopsis. Seedling phenotypes under the given conditions are shown in sections A to H, wild type on the left, heterozygous mutant on the right. A to C, Three-day-old light-grown seedlings on 0.5× MS (A), 0.5× MS supplemented with 10 μm of sirtinol (B), or 100 nm of the synthetic auxin 2,4-D (C). D, Seven-day-old light-grown seedlings on 0.5× MS. E to H, Three-day-old dark-grown seedlings on 0.5× MS (E), 0.5× MS supplemented with 2 μm sirtinol (F), 250 nm 2,4-D, or 2 μm of the ethylene precursor ACC (G). Later stage phenotypes are shown in I and J, wild type on the left, heterozygous mutant in the center, and heterozygous recapitulation line on the right. I, Fully formed rosette leaf stage. J, Mature plants. Hormone response curves for IAA and ACC are provided in sections K and L, respectively.