Unrooted neighbor-joining tree for the NEC4-related genes. Highly homologous (E scores < 1e−20) protein sequences were aligned using ClustalW and an unrooted nonjoining tree was developed. The NEC4, tomato XEGIP, and wheat TAXI-1 gene products (marked by asterisks) have been shown to inhibit fungal hemicellulases. Three plant families, Solanaceae, Poaceae, and Fabaceae, are indicated by dashed circles. The protein sequences were derived from the following gene sequences: LxS NEC4 (ornamental tobacco), accession number AY898762; StXEGIP (potato), AY321357; LeXEGIP (tomato), AY155579; DcEDGP (carrot), D14550; Mxd EDGP (apple), AY347867; At1g03220 (Arabidopsis), AY085016; At1g03230 (Arabidopsis), AF332411; At5g19100 (Arabidopsis), NM_121915; At5g19110 (Arabidopsis), NM_121916; At5g19120 (Arabidopsis), AY055793; At5g48430 (Arabidopsis), AB020745; OsjNb0006J12.8 (rice), AC120991.3; OsjNb0006J12.9 (rice), AC120991.3; OsjNb0006J12.10 (rice), AC120991.3; OsjNb0006J12.11 (rice), AC120991.3; OsjNb0504E02.3 (rice), NM_190067; OsjNb0504E02.6 (rice), NM_190069; OsjNb0504E02.8 (rice), NM_190071; OsjNb0504E02.9 (rice), NM_190072; OsjNb0504E02.10 (rice), NM_190073; OsjNb0504E02.13 (rice), NM_190075; OsjNb0504E02.14 (rice), NM_190076; OsjNb0504E02.16 (rice), NM_190078; OsjNbP0659B09.5 (rice), AP005886.3; CaEDGP (chickpea), AJ299060; Lupal conglutin γ clone 32 (white lupin), AJ297490; Lupal conglutin γ clone 48 (white lupin), AJ297568; Lupan conglutin γa (narrow leaved blue lupin), X65601; Lupan conglutin γb (narrow leaved blue lupin), X65601; Gm7SBg (soybean), AB084260; Gm7SBg108 (soybean), X16469; Gm7SBPM5 (soybean), U59425.1; Gm7SBgW1 (soybean), D16107; HvXI-1 (barley), AJ581529; ScXI-1 (rye), AJ581532; TaXI-1 (wheat), AB114626; TaXI-2 (wheat), AJ438880; TaXI-3 (wheat), AB178471; and TaXI-4 (wheat), AB114628.