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Mutation of the HSE or DRE sequences eliminates synergism between HaHSFA9 and HaDREB2. Sunflower embryos were bombarded with the −289(G1):LUC reporter gene constructs indicated above each section and with the effector plasmids indicated at the bottom. Mutated cis-elements are crossed on the construct maps. Sample sizes, statistical comparisons, and symbols for effector plasmids are described in Figures 1 and 3. The results of statistical analyses are detailed in Supplemental Table I.
