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. 2005 Nov;139(3):1518–1528. doi: 10.1104/pp.105.068684

Table II.

The effect of Se on S metabolism

Genes encoding sulfate transporters and genes for the synthesis of Cys, Met, and glutathione were selected from the transcriptome data of wild-type (Ws) and CpNifS overexpressor (NifS55 and NifS38) plants, described as set 1 and set 2 in Table I. The data of 24 h no-S treatment (−S) and the control S-replete culture at the same time point (+S) appear as references (set A and set B; Maruyama-Nakashita et al., 2005; http://arabidopsis.org/info/expression/ATGenExpress.jsp). Columbia-0 ecotype (Col) was used for the S limitation experiment. The up- and down-regulation by Se or by S limitation were calculated as relative ratios of normalized signals (Se/MS and −S/+S), and displayed using the same superscript letters as in Table I. The relative ratios are calculated for the pairs having “present” call at least in one sample.

Affy Code
AGI Locus
Annotation
Gene Name
Set 1
Set 2
Set A
Set B
Ws
NifS55
Ws
Nifs38
Col
Col
Se MS Se/MS Se MS Se/MS Se MS Se/MS Se MS Se/MS −S +S −S/+S −S +S −S/+S
Sulfate transport
255105_at AT4G08620 Sulfate transporter SULTR1;1 2.69 P 0.29 P 9.33a 2.45 P 0.59 P 4.15b 2.40 P 0.21 P 11.41a 3.33 P 0.25 P 13.59a 1.41 P 0.16 P 9.04a 1.90 P 0.13 P 15.10a
262133_at AT1G78000 Sulfate transporter SULTR1;2 1.15 P 0.65 P 1.76c 1.08 P 0.98 P 1.10 1.14 P 0.69 P 1.64c 1.23 P 0.69 P 1.78c 1.15 P 0.73 P 1.58c 1.02 P 0.72 P 1.42c
255958_at AT1G22150 Sulfate transporter SULTR1;3 0.59 A 1.74 A 0.53 A 0.49 A 0.64 A 0.27 A 0.54 A 0.90 A 1.01 A 0.77 A 0.74 A 0.20 A
250475_at AT5G10180 Sulfate transporter SULTR2;1 1.80 P 0.34 P 5.35a 1.79 P 0.23 P 7.76a 2.03 P 0.27 P 7.59a 1.66 P 0.27 P 6.11a 2.33 P 0.24 P 9.60a 2.75 P 0.31 P 8.94a
262134_at AT1G77990 Sulfate transporter SULTR2;2 1.16 P 1.14 P 1.02 1.31 P 1.04 P 1.26 0.97 P 0.89 P 1.10 1.03 P 0.72 A 1.42c 1.16 P 0.87 P 1.34c 0.85 P 0.76 A 1.12
246310_at AT3G51895 Sulfate transporter SULTR3;1 1.05 P 1.03 P 1.02 0.74 P 0.66 P 1.11 1.39 P 1.05 P 1.33c 0.87 P 0.89 P 0.98 1.42 P 0.97 P 1.47c 1.10 P 0.65 P 1.69c
255443_at AT4G02700 Sulfate transporter SULTR3;2 0.83 A 0.75 A 0.80 A 1.01 A 1.10 A 1.00 A 0.99 A 1.06 A 1.16 A 1.00 A 1.07 A 0.75 M
264901_at AT1G23090 Sulfate transporter SULTR3;3 0.72 P 1.41 P 0.51d 1.36 P 1.82 P 0.75d 0.78 P 1.32 P 0.59d 1.16 P 1.43 P 0.81 0.83 P 0.84 P 0.99 0.83 P 0.84 P 0.98
258287_at AT3G15990 Sulfate transporter SULTR3;4 1.45 P 0.91 P 1.59c 1.27 P 1.07 P 1.19 1.62 P 1.14 P 1.42c 1.45 P 0.93 P 1.56c 0.69 P 0.46 P 1.50c 0.66 P 0.48 P 1.37c
245912_at AT5G19600 Sulfate transporter SULTR3;5 0.88 P 0.96 P 0.92 0.93 P 1.02 P 0.91 0.88 P 0.98 P 0.89 0.88 P 1.19 P 0.74d 1.13 P 1.36 P 0.83 1.19 P 1.28 P 0.92
245855_at AT5G13550 Sulfate transporter SULTR4;1 1.33 P 0.81 P 1.65c 1.24 P 0.88 P 1.42c 1.24 P 0.73 P 1.71c 1.26 P 0.63 P 2.00c 1.26 P 0.81 P 1.56c 1.12 P 0.60 P 1.88c
256244_at AT3G12520 Sulfate transporter SULTR4;2 1.90 P 0.58 P 3.30b 2.01 P 0.67 P 2.99b 1.76 P 0.67 P 2.63b 2.05 P 0.56 P 3.65b 1.40 P 0.34 P 4.18b 1.33 P 0.41 P 3.27b
Sulfate reduction
256835_at AT3G22890 ATP sulfurylase APS1 1.05 P 0.65 P 1.60c 0.96 P 0.62 P 1.54 0.99 P 0.60 P 1.67c 1.01 P 0.62 P 1.61c 1.70 P 1.32 P 1.29 1.97 P 1.79 P 1.10
255785_at AT1G19920 ATP sulfurylase APS2 0.62 P 1.12 P 0.56d 0.75 P 1.00 P 0.75 0.69 P 1.27 P 0.54d 0.72 P 1.17 P 0.61d 0.86 P 1.36 P 0.63d 1.01 P 1.28 P 0.79
245254_at AT4G14680 ATP sulfurylase APS3 1.99 P 0.37 A 5.40a 1.27 P 0.32 A 4.00b 2.12 P 0.33 A 6.34a 1.69 P 0.47 M 3.59b 1.19 P 0.62 A 1.94c 1.39 P 0.81 P 1.72c
249112_at AT5G43780 ATP sulfurylase APS4 0.27 P 1.14 P 0.23e 0.30 P 1.14 P 0.27e 0.26 P 1.03 P 0.25e 0.28 P 1.04 P 0.27e 0.81 P 1.14 P 0.72d 0.97 P 1.46 P 0.67d
255284_at AT4G04610 APS reductase APR1 1.02 P 0.32 P 3.24b 1.08 P 0.38 P 2.87b 0.94 P 0.32 P 2.96b 1.33 P 0.35 P 3.80b 1.02 P 0.99 P 1.03 1.22 P 1.38 P 0.88
264745_at AT1G62180 APS reductase APR2 1.11 P 0.50 P 2.23b 1.18 P 0.40 P 2.93b 1.16 P 0.39 P 2.94b 1.28 P 0.43 P 2.97b 1.38 P 0.77 P 1.79c 1.49 P 0.89 P 1.67c
254343_at AT4G21990 APS reductase APR3 2.10 P 0.42 P 5.06a 1.91 P 0.47 P 4.11b 2.41 P 0.48 P 5.07a 2.30 P 0.44 P 5.18a 1.60 P 0.57 P 2.82b 1.38 P 0.62 P 2.21b
267112_at AT2G14750 APS kinase AKN1 1.11 P 0.89 P 1.26 1.10 P 0.95 P 1.16 0.76 P 0.73 P 1.03 1.05 P 0.68 P 1.55c 1.17 P 1.09 P 1.07 0.81 P 1.47 P 0.55d
252870_at AT4G39940 APS kinase AKN2 1.17 P 0.99 P 1.19 1.10 P 1.01 P 1.09 0.99 P 0.76 P 1.30 1.02 P 0.80 P 1.27 0.83 P 1.63 P 0.51d 0.67 P 2.27 P 0.30e
259339_at AT3G03900 APS kinase AKN3 0.74 P 1.08 P 0.69d 0.94 P 0.98 P 0.96 1.14 P 0.95 P 1.20 1.11 P 0.98 P 1.13 1.18 P 1.22 P 0.97 0.95 P 1.02 P 0.93
247005_at AT5G67520 APS kinase AKN4 0.98 P 0.93 P 1.05 1.02 P 1.15 P 0.89 0.98 P 0.98 P 1.00 0.89 P 0.88 P 1.01 1.20 P 1.63 P 0.74d 1.31 P 1.08 P 1.21
250846_at AT5G04590 Sulfite reductase SIR 1.03 P 0.90 P 1.15 1.21 P 0.82 P 1.47c 1.21 P 0.90 P 1.35c 1.09 P 0.85 P 1.29 0.93 P 1.05 P 0.88 0.97 P 1.21 P 0.80
Cys synthesis
245286_at AT4G14880 O-acetylserine (thiol) lyase Bsas1;1 1.00 P 1.11 P 0.90 1.00 P 1.35 P 0.74d 1.03 P 1.10 P 0.93 0.98 P 1.11 P 0.88 0.77 P 0.90 P 0.85 0.72 P 0.79 P 0.92
256930_at AT3G22460 O-acetylserine (thiol) lyase Bsas1;4 0.95 P 1.54 P 0.62d 1.23 P 2.17 P 0.57d 0.69 P 1.45 P 0.48e 1.05 P 1.17 P 0.90 0.81 P 0.81 P 1.00 0.94 P 0.92 P 1.03
260566_at AT2G43750 O-acetylserine (thiol) lyase Bsas2;1 1.38 P 1.00 P 1.38c 1.12 P 0.90 P 1.25 1.49 P 0.95 P 1.57c 1.21 P 1.00 P 1.21 1.01 P 0.87 P 1.16 0.87 P 0.91 P 0.96
251487_at AT3G59760 O-acetylserine (thiol) lyase Bsas2;2 1.01 P 0.97 P 1.04 0.96 P 1.00 P 0.96 0.94 P 1.09 P 0.86 1.06 P 1.00 P 1.06 0.85 P 1.11 P 0.77 0.84 P 1.01 P 0.83
251322_at AT3G61440 O-acetylserine (thiol) lyase Bsas3;1 1.13 P 1.18 P 0.95 1.02 P 1.24 P 0.82 0.98 P 1.11 P 0.88 0.98 P 1.04 P 0.94 0.94 P 0.94 P 1.00 0.72 P 0.88 P 0.82
259094_at AT3G04940 O-acetylserine (thiol) lyase Bsas4;1 1.03 P 1.17 P 0.88 0.84 P 0.97 P 0.87 0.86 P 1.07 P 0.81 0.76 P 0.90 P 0.84 1.15 P 0.90 P 1.27 1.03 P 1.31 P 0.79
246701_at AT5G28020 O-acetylserine (thiol) lyase Bsas4;2 1.75 P 0.98 P 1.78c 1.58 P 1.15 P 1.37c 1.66 P 1.02 P 1.62c 1.51 P 0.87 P 1.74c 0.81 P 0.83 P 0.98 0.86 P 0.65 P 1.34c
246700_at AT5G28030 O-acetylserine (thiol) lyase Bsas4;3 0.24 A 0.14 A 1.77 A 1.01 A 1.60 A 0.38 A 1.06 A 0.88 A 1.24 A 1.06 A 0.99 A 0.18 A
259172_at AT3G03630 O-acetylserine (thiol) lyase Bsas5;1 1.04 A 0.71 P 1.47c 0.93 A 1.00 A 0.93 1.00 A 1.14 P 0.88 1.05 A 0.85 A 1.23 0.91 A 0.93 A 0.98 1.57 A 1.09 P 1.44c
247982_at AT5G56760 Ser acetyltransferase Serat1;1 0.92 P 1.04 P 0.89 0.91 P 1.00 P 0.91 0.87 P 1.00 P 0.87 0.94 P 0.91 P 1.04 1.99 P 1.83 P 1.09 2.00 P 2.04 P 0.98
260602_at AT1G55920 Ser acetyltransferase Serat2;1 1.46 P 1.22 P 1.20 1.28 P 1.52 P 0.84 1.11 P 0.94 P 1.18 1.06 P 0.86 P 1.23 0.82 P 0.63 P 1.30 0.48 P 0.48 P 1.01
257194_at AT3G13110 Ser acetyltransferase Serat2;2 1.23 P 1.17 P 1.05 0.93 P 1.23 P 0.76 0.78 P 0.84 P 0.93 0.75 P 0.90 P 0.83 0.98 P 1.07 P 0.91 1.02 P 1.13 P 0.90
264594_at AT2G17640 Ser acetyltransferase Serat3;1 2.24 P 0.98 P 2.29b 2.22 P 0.95 P 2.34b 1.86 P 0.97 P 1.93c 1.93 P 0.72 P 2.67b 1.47 P 0.79 P 1.87c 1.02 P 0.51 P 2.03b
GSH and Met synthesis
254270_at AT4G23100 γ-Glutamylcysteine synthetase GSH1 1.02 P 1.52 P 0.67d 1.07 P 1.67 P 0.64d 0.92 P 1.45 P 0.64d 0.92 P 1.38 P 0.66d 0.75 P 0.89 P 0.84 0.69 P 0.98 P 0.71d
246785_at AT5G27380 Glutathione synthetase GSH2 1.22 P 1.16 P 1.05 1.12 P 1.03 P 1.09 1.11 P 0.97 P 1.14 1.07 P 0.90 P 1.19 0.70 P 0.63 P 1.10 0.66 P 0.82 P 0.80
259279_at AT3G01120 Cystathionine γ-synthase CGS 0.97 P 1.12 P 0.87 0.85 P 1.07 P 0.80 0.90 P 1.03 P 0.87 0.78 P 1.12 P 0.70d 0.83 P 1.04 P 0.80 0.73 P 1.05 P 0.70d
256531_at AT1G33320 Cystathionine γ-synthase, putative 0.79 A 1.52 A 0.96 A 1.40 A 0.78 A 0.72 A 1.04 A 0.90 A 1.25 A 1.43 A 0.90 A 1.29 A
251666_at AT3G57050 Cystathionine β-lyase CBL 1.19 P 1.19 P 0.99 1.01 P 1.03 P 0.98 0.87 P 0.99 P 0.88 0.91 P 0.94 P 0.98 0.83 P 1.06 P 0.78 0.95 P 1.32 P 0.72d
246185_at AT5G20980 Met synthase 0.98 P 0.86 P 1.14 0.81 P 0.78 P 1.05 1.02 P 0.88 P 1.17 0.95 P 1.17 P 0.81 1.44 P 1.54 P 0.94 1.41 P 1.22 P 1.16
259343_s_at AT3G03780 Met synthase 0.95 P 0.93 P 1.02 0.88 P 0.94 P 0.94 0.99 P 1.01 P 0.97 0.99 P 1.01 P 0.98 1.80 P 1.74 P 1.04 1.45 P 1.61 P 0.90
Met metabolism
261957_at AT1G64660 Cystathionine γ-lyase, putative 1.63 P 1.06 P 1.54c 1.69 P 1.09 P 1.55c 1.77 P 0.94 P 1.88c 1.56 P 0.94 P 1.66c 0.45 P 0.26 P 1.76c 0.38 P 0.21 P 1.85c
260594_at AT1G55880 Cystathionine β-synthase, putative 0.98 P 1.32 P 0.75d 1.00 P 1.11 P 0.90 0.89 P 1.09 P 0.81 0.80 P 0.94 A 0.85 1.00 P 0.80 A 1.26 1.02 A 1.04 P 0.98
260913_at AT1G02500 S-adenosylmethionine synthetase SAM1 1.06 P 1.16 P 0.92 0.94 P 1.31 P 0.71d 1.15 P 1.09 P 1.05 0.93 P 1.19 P 0.78 0.94P 0.88 P 1.06 0.75 P 0.88 P 0.85
255552_at AT4G01850 S-adenosylmethionine synthetase SAM2 1.23 P 1.38 P 0.89 0.99 P 1.25 P 0.79 0.97 P 1.10 P 0.88 0.90 P 1.15 P 0.78 1.01P 0.77 P 1.32 0.76 P 0.86 P 0.89
263838_at AT2G36880 S-adenosylmethionine synthetase SAM3 1.12 P 1.24 P 0.90 0.97 P 1.19 P 0.82 0.93 P 1.06 P 0.88 0.86 P 0.99 P 0.87 1.09P 0.87 P 1.26 0.85 P 1.01 P 0.84
258415_at AT3G17390 S-adenosylmethionine synthetase 1.46 P 1.05 P 1.39c 1.16 P 0.89 P 1.31 1.34 P 0.94 P 1.42c 1.06 P 0.89 P 1.19 1.26P 0.79 P 1.59c 0.95 P 0.87 P 1.10
245356_at AT4G13940 S-adenosylhomocysteine hydrolase 1.22 P 1.22 P 1.00 0.94 P 1.16 P 0.81 1.18 P 1.05 P 1.12 0.91 P 1.12 P 0.82 0.95P 0.78 P 1.21 0.71 P 0.84 P 0.84
257173_at AT3G23810 S-adenosylhomocysteine hydrolase 0.86 P 1.00 P 0.86 0.82 P 0.92 P 0.88 0.93 P 1.00 P 0.93 0.82 P 1.13 P 0.73d 1.80P 1.55 P 1.16 1.60 P 1.71 P 0.94
258075_at AT3G25900 Homocysteine S-methyltransferase HMT-1 1.13 P 0.89 P 1.28 1.07 P 1.02 P 1.05 0.98 P 0.83 P 1.18 0.89 P 0.83 P 1.08 1.07P 0.86 P 1.25 1.20 P 1.21 P 0.99
251175_at AT3G63250 Homocysteine S-methyltransferase HMT-2 1.17 P 1.15 P 1.02 1.05 P 0.95 P 1.11 1.11 P 1.17 P 0.95 0.91 P 1.08 P 0.84 0.71P 0.76 P 0.93 0.86 P 0.84 P 1.03
258322_at AT3G22740 Homocysteine S-methyltransferase HMT-3 1.00 P 1.10 P 0.90 0.64 P 1.58 P 0.41e 1.01 A 0.97 P 1.03 0.73 A 1.33 P 0.55d 0.89A 2.02 P 0.44e 0.93 A 2.22 P 0.42e
Se-binding protein
245285_s_at AT4G14030 Se-binding protein, putative 1.102 P 0.446 P 2.47b 1.165 P 0.635 P 1.83c 0.986 P 0.573 P 1.72c 1.28 P 0.49 P 2.61b 1.314P 1.081 P 1.22 1.014 P 0.882 P 1.15
245285_s_at AT4G14040 Se-binding protein, putative
257197_at AT3G23800 Se-binding protein, putative 0.59 P 1.369 P 0.43e 0.743 P 1.386 P 0.54d 0.505 P 1.22 P 0.41e 0.64 P 1.139 P 0.56d 0.861P 1.215 P 0.71d 0.839 P 1.629 P 0.52d