Table 1. Comparative analysis between His-TAC and Y2H methods to efficiently reveal specific TRX/target complexes in trx1Δtrx2Δ yeast mutants.

	Affinity chromatography*				Yeast two-hybrid†
Targets	TRX1	TRX2	Ath2	Ath3	TRX1	TRX2	Ath2	Ath3
AHP1	+++	+++	+++	+++	++	+++	−	++
TSA1	++	+	+	NI	−	++++	−	NT
MET16	+	+	+	NI	+++	++	++	−
MSRA	NI	NI	NI	NI	+	++	+/−	+/−

All TRX baits are mutated and carry a CXXS active site. NT, not tested; Ath2, AtTrxh2; Ath3, AtTrxh3.

^*Plus signs (+, ++, +++), spot density as observed on 2D gels. NI, the corresponding target was not identified among the 2D spots analyzed.

^†Plus signs (+, ++, +++), the level of two-hybrid interactions; −, no interaction occurred between partners tested; +/−, a faint but reproducible interaction.