Fig. 2.

Relative amounts of CEF-related enzymes and transcripts of mesophyll cells to bundle-sheath cells. (a) Immunoblot analysis of NDH-H, PGR5, and cytochrome f. (b-d) Densitometric analysis of immunoblots using polyclonal antibodies against NDH-H (b), PGR5 (c), and cytochrome f (d) proteins. Each value represents the relative intensity of mesophyll preparations to bundle-sheath preparations. Data are means ± SD (n = 3). (e) Relative pgr5 gene transcripts of mesophyll preparations to bundle-sheath preparations. The amounts of pgr5 gene transcripts were estimated by qPCR as described in Materials and Methods, normalized to the corresponding actin mRNA levels. Data are the means ± SD (n = 5).