Fig. 4.

In vitro LRRK2 kinase assay. (A) Immunoprecipitated LRRK2 preparation derived from transfected HEK-293T cells as analyzed by SDS/PAGE and subsequent silver stain. (B) Assessment of WT or mutant LRRK2 protein levels by Western blot using anti-c-myc antibody. (C) In vitro kinase activity of recombinant WT or mutant LRRK2 against biotinylated MBP. Incorporated ³²P in MBP was assessed by liquid scintillation, and results are representative of at least three independent experiments. Error bars represent mean ± SEM. n.s., not significant. ^*, P < 0.05 assessed by two-tailed unpaired Student's t test.