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Autophosphorylation of LRRK2. (A) Autoradiogram of incorporated 32P in mutant or WT LRRK2 recombinant protein resolved by SDS/PAGE. (B) LRRK2 protein levels were determined by Western blot analysis by using anti-c-myc antibody. (C) Normalization of incorporated 32P compared with LRRK2 protein content. Data represent at least three independent experiments. a.u., arbitrary units. Error bars are mean ± SEM. *, P < 0.01; **, P < 0.001 assessed by two-tailed unpaired Student's t test.
