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. 2005 Nov 7;102(46):16584–16589. doi: 10.1073/pnas.0508306102

Fig. 2.

Fig. 2.

GTP[γS] binding to GoLoco protein/Gαi-1 complexes. (A) Full-length LGN/Gαi-1 (triangles), LGN/Gαi-1/NuMA (squares) complexes (50 nM), and free Gαi-1 (circles) (200 nM) were used to examine intrinsic (filled symbols) and 200 nM Ric-8A-stimulated (open symbols) rates of GTP[γS] binding. (B) LGNshort/Gαi-1 (triangles), AGS3short/Gαi-1 (squares) complexes (50 nM), and free Gαi-1 (circles) (200 nM) were used to examine intrinsic (filled symbols) and 200 nM Ric-8A-stimulated (open symbols) rates of GTP[γS] binding. Each reaction contained 10 mM MgCl2 and 10 μM[35S]GTP[γS] (10,000 cpm/pmol). Results are shown as the mean of triplicate experiments ± standard deviation.