Table 1. GTP[γS] binding to GoLoco protein/Gαi-1 complexes.
| Kobs, min-1 | Ric-8A-stimulated Kobs, min-1 | Percent GoLoco inhibition (intrinsic) | Percent GoLoco inhibition (Ric-8A-stimulated) | Fold Ric-8A stimulation | |
|---|---|---|---|---|---|
| Gαi-1 | 0.048 ± 0.006 | 0.253 ± 0.004 | — | — | 5.3 |
| Gαi/LGN | 0.029 ± 0.005 | 0.202 ± 0.011 | 39.6 | 20.2 | 7.0 |
| Gαi/LGN/NuMA | 0.034 ± 0.007 | 0.153 ± 0.012 | 29.2 | 39.5 | 4.5 |
| Gαi/LGNshort | 0.031 ± 0.003 | 0.155 ± 0.005 | 35.4 | 38.7 | 5.0 |
| Gαi/AGS3short | 0.039 ± 0.004 | 0.212 ± 0.006 | 18.8 | 16.2 | 5.4 |
Reactions rates (Kobs) were calculated from single exponential rate equations generated by using the origin 6.0 (Microcal Software) curve-fitting program.