Fig. 3.

Lack of TrkA^NGFR protein expression in the cornea of keratoconus patients. (A) Representative immunohistochemical staining of TrkA^NGFR in control (a) and keratoconus (b) corneas. Higher magnification shows the lack of TrkA^NGFR in corneal cells, including epithelium (compare c with d) and keratocytes (compare e with f). Sections were counterstained with Harri's hematoxylin. (B) Western blot analysis of corneal protein extracts. The immunoreactive levels of TrkA^NGFR (arrow) are shown for control, Fuchs' dystrophy, bullous, and keratoconus corneas. In the first lane (Control), the lower band is a nonspecific immunoreactive band.