Fig. 5.

Overexpression of Sp3 short isoform(s) down-regulates TrkA^NGFR expression in corneal keratocytes. (A) A panel of confocal images of cultured primary corneal keratocytes transfected with HA-Sp3 construct. Cells were double stained for TrkA^NGFR (red) and the HA-tag (green). Keratocytes that overexpress Sp3 short isoform(s) have nuclear staining (green) and reduced cytoplasmic TrkA^NGFR immunofluorescence. (B) Keratocytes that overexpress control plasmid (EGFP-N1 vector) do not have altered TrkA^NGFR immunostaining.