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. 2005 Nov 7;102(46):16795–16800. doi: 10.1073/pnas.0508516102

Fig. 6.

Fig. 6.

Sp3 down-regulates transcription from TrkANGFR proximal promoter. (A) Western blot of protein extracts of HACAT cells transfected with the HA-Sp3 construct. Protein levels of TrkANGFR are reduced only in extracts of cells that overexpress the HA-Sp3 short isoform(s) compared with cells that overexpress the empty HA vector or the control GFP vector (EGFP-N1). Protein loading was monitored by reprobing the nitrocellulose with β-actin antibody. (B) HACAT cells were cotransfected with pTrkA-Luc reporter construct and increasing amounts of HA-Sp3 vector. Luciferase activity was measured after 48 h. Results, which are representative of three independent experiments performed in triplicate, are reported as percent of luciferase activity (±SD) compared with the transfections carried out in the absence of HA-Sp3.