18S	18S ribosomal RNA
2D	two-dimensional
2D-GelyP	Cells cultured on a surface coated with 0.1% gelatin-peptone surface
2D-Coll	Cells cultured on a coated surface with a thin layer of type I collagen surface
2D-TC	Cells cultured on standard tissue culture-treated plastic surfaces
3D	three-dimensional
3D-Coll	Three-dimensional sandwich method of cultivation in collagen type I
3Rs	Reduction, Refinement, and Replacement
Alb	Albumin
AML12	Mouse hepatocytes alpha mouse liver 12
Actb	β-actin
B2M	Beta-2 microglobulin
BK	BestKeeper index
cDNA	Complementary deoxyribonucleic acid
Ct	Cycle threshold
ΔCt	delta Ct
ΔΔCt	delta delta Ct
CV	coefficient of variation
DMEM	Dulbecco’s Modified Eagle’s Medium
Gapdh	Glyceraldehyde-3-phosphate dehydrogenase
HKG	Housekeeping genes
Hmbs	Hydroxymethylbilane synthase
Hprt	Hypoxanthine guanine phosphoribosyl transferase
ITS	Insulin–transferrin–selenium
MHC I	Major histocompatibility complex class I
PBS	Phosphate-buffered saline
Ppia	Peptidylprolyl isomerase A
RNA	Ribonucleic acid
Rplp0	Ribosomal protein, large, P0
RT-qPCR	Reverse transcription quantitative polymerase chain reaction
SD	standard deviation
SEM	Standard error of the mean
TBP	TATA-box binding protein
Ywhaz	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide