Figure 10.

p75-mediated apoptosis can be restored in nrif−/− sympathetic neurons by expression of WT NRIF, but not K19R NRIF. Sympathetic neurons from nrif−/− mice were isolated, electroporated with GFP, GFP-NRIF, or GFP-K19R NRIF, and cultured in 20 ng/ml NGF for 2 days. The NGF was then removed, the neurons switched to media containing anti-NGF together with 12.5 mM KCl to promote survival and treated with (black bars) or without (empty bars) 100 ng/ml BDNF for 48 h. The cells were then fixed and GFP-positive neurons were evaluated for apoptotic nuclei by DAPI staining. Depicted are the mean±s.e.m. (n=3; ^*P<0.008).