Figure 4.

NRIF polyubiquitination is undetectable in traf6 null mice. (A) WT and traf6 KO mouse brain were homogenized in SDS lysis buffer, immunoprecipitated with anti-NRIF and immunoblotted with anti-ubiquitin (upper panel) or anti-NRIF (lower panel). (B) WT and traf6 KO mouse brains were homogenized in Triton lysis buffer, NRIF was immunoprecipitated and Western blotted with NRIF, TRAF6 or p62 antiserum to examine their interaction (upper panels). The same brain lysates were also Western blotted with anti-NRIF, TRAF6 and p62 to confirm the expression of these proteins (lower panels).