Figure 8.

TRAF6 is necessary for NRIF nuclear localization in vivo. Whole brains were isolated from postnatal day 2 traf6 WT and KO mice, homogenized in RIPA buffer, then NRIF immunoprecipitated and the precipitates immunoblotted with NRIF antibody (upper panel). Nuclei were isolated from these tissues and NRIF immunoprecipitated. The precipitates were then subjected to Western blotting using an NRIF antibody (middle panel). Note the reduced presence of NRIF in traf6−/− nuclei relative to the WT. Nuclear extract (NE) and post nuclear supernatant (PNS) from WT and KO mouse brain were subjected to HDAC1 Western blot analysis to confirm the isolation of the nuclei, as well as serving as a loading control for the nuclear extracts (lower panel).