Figure 4.
The CH1 domain is absolutely required for C-TAD transactivation function but is less essential for HIF target genes. (A, B) Deletion of CBPflox and (A) p300flox (B) in MEFs following infection with Cre-expressing adenovirus. MEF genotypes, days post infection, and allele-specific products derived from semiquantitative PCR of genomic DNA are indicated. (C, D) Comparable growth curves for tri-ΔCH1/flox and flox MEFs with or without Cre-adenovirus (Ad Cre) infection. (E, F) Normalized activity of Gal-HIF-1α, Gal-HIF-2α, and Gal-Myb in transiently transfected Δflox and tri-ΔCH1/Δflox MEFs (mean±s.e.m., N=3). (G, H) WT CBP contributes marginally to residual hypoxia-inducible gene expression in triple-ΔCH1 MEFs. qRT–PCR analysis of control flox and tri-ΔCH1/flox MEFs±Cre-adenovirus infection. Slc2a1 (G) and Pfkfb3 (H), tested under normoxia and hypoxia, normalized to β-actin mRNA (mean±s.e.m., N=2–3).