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. 2005 Oct 20;24(22):3846–3858. doi: 10.1038/sj.emboj.7600846

Figure 5.

Figure 5

Markedly reduced recruitment of CBPΔCH1 and p300ΔCH1 to HIF-binding sites does not strongly correlate with HIF-responsive transcription. (A–C) Quantitative ChIP assays of Slc2a1, Pfkfb3, and Hig1, using WT, CBPΔCH1/ΔCH1, and p300ΔCH1/ΔCH1 MEFs treated for 2 h with ethanol vehicle (EtOH) or DP/MG132/ALLN (DP) (mean±s.e.m., N=3 independent experiments). Control (NRS) and specific (anti-CBP, anti-p300) immunoprecipitation antisera are indicated. DP-dependent ChIP signal was determined by subtracting the EtOH signal from the DP signal after normalizing to the input DNA signal. (D–F) qRT–PCR analysis of HIF-target gene expression in Δflox #2 and tri-ΔCH1/Δflox #2 MEFs after 6 h DP/MG132/ALLN, normalized to β-actin mRNA (mean±s.e.m., N=3).