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. 2005 Oct 27;24(22):3927–3939. doi: 10.1038/sj.emboj.7600848

Figure 1.

Figure 1

Transcription-mediated siRNA and RNA duplex siRNA both result in >90% depletion of CENP-F. (A–D) CENP-F repression was obtained by transcription-mediated siRNA and RNA duplex siRNA. HeLa cells were transfected with (A) the corresponding siRNA plasmids along with pCD20 that encodes the cell surface marker CD20 or (B) siRNA duplex RNAs and a luciferase–Cy3 RNA duplex. Accumulated CENP-F levels were visualized by (C) immunoblotting whole-cell lysates and (D) immunofluorescence 48 h after transfection.