Figure 1.
Experimental approach and removal of CPDs upon PR. (A) Graphical representation of the experimental design. For each dose and time point, labelled cDNAs derived from irradiated, PR and non-PR samples (upper and lower horizontal bars) were hybridized to unirradiated, non-PR material from the same time point (middle bar). D: dark; L: light. (B) CPDs were detected by indirect immunofluorescence; nuclei were visualized by DAPI staining. UV dose (No UV or 8 J/m2) and PR status (light or dark) are indicated on the bottom; time after the 1 h PR period is indicated on top.