Skip to main content
. 2005 Oct 27;24(22):3906–3916. doi: 10.1038/sj.emboj.7600852

Figure 7.

Figure 7

Reverse NAIS analysis confirms a direct interaction between 2′-OH of G844 in D5 and the pentaloop of D3. (A) The autoradiograph of representative high-resolution sequencing gels after iodine cleavage of unreacted exD123 RNAs and branched products. Selection step (branching reaction) was carried out in the presence of wild-type and mutant unlabeled D56 RNAs and 3′-end labeled wild-type exD123 transcribed in the presence of 2,6-DAP. Numbers on the left indicate adenosine positions in exD123. (B) A diagram comparing relative intensity of bands corresponding to A617 and A618 in precursor (0) and branched products.