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. 2005 Oct 27;24(22):3940–3951. doi: 10.1038/sj.emboj.7600854

Figure 1.

Figure 1

Identification of Cdt2 as an interacting protein with the Pcu4–CSN complex. (A) Co-IP of Cdt2-HA and Csn1-Myc. Csn1-Myc IP (α-Myc): In a wild-type background (Wt, lane 3), co-precipitated Cdt2-HA was detected with α-HA. The interaction is lost in csn2-d and ddb1-d (lanes 4 and 6) but not csn5-d (lane 5) extracts. *Indicates a second Cdt2-HA-dependent band we assume to be a degradation product. The intensity of this band varies between experiments. (B) Co-IP of Cdt2-HA and Ddb1-Myc. Ddb1-Myc was IPed with α-Myc. Cdt2-HA was detected with α-HA. The interaction was not affected by loss of other Pcu4–CSN subunits. (C) Co-precipitation of Ddb1-Myc and Csn1-TAP. Csn1-TAP was precipitated with IgG-coated Dynabeads. Ddb1-Myc was detected with α-Myc. Loss of cdt2 did not prevent the interaction between Csn1-TAP and Ddb1-Myc.