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. 2005 Nov 10;24(22):3974–3983. doi: 10.1038/sj.emboj.7600859

Figure 4.

Figure 4

Effect of nucleotides on the distribution of single-pair FRET efficiency of subunit F. (A–D) Distribution of the single-pair FRET efficiency of the double-labeled subunit F (A55C, H89C) in the presence or absence of various nucleotides. (A) ATP (2 mM) in the presence of an ATP regeneration system (n=72). (B) AMP-PNP (2 mM) (n=25), (C) 2 mM ADP (n=40), (D) in the absence of nucleotide (n=25). (E, F) Distribution of the single-pair FRET efficiency of the double-labeled subunit F (V2C, A55C) in the V1 complex. (E) ATP (2 mM) in the presence of ATP, a regeneration system (n=27). (F) ADP (2 mM) (n=30). Measurement of single-pair FRET efficiency and construction of mutant subunit F are described in Materials and methods.