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. 2005 Nov 15;19(22):2695–2704. doi: 10.1101/gad.1367605

Figure 5.

Figure 5.

ChIP analysis of Snf2 and Ada1 association to the SRG1 promoter. (A) ChIP analysis of Snf2 was performed on wild-type (FY2470), cha4Δ (FY2469), and srg1-25 (FY2468) strains expressing Snf2-myc and an untagged control strain (FY1350). Snf2-myc was immunoprecipitated with anti-myc A14 antibody from chromatin isolated from cells that were grown in SD + ser medium (+serine) and from cells that were shifted from SD + ser to SD medium for 25 min (–serine). The amounts of SRG1 UAS and SER3 UAS that were amplified from immunoprecipitated DNA are expressed as percentages of the amounts of input DNA. Each bar represents the average and standard error from three independent experiments. (B) ChIP analysis of Ada1. Ada1 was immunoprecipitated with anti-Ada1 antibody from the same chromatin that is described in A with the exception of the untagged control strain, which was replaced by an ada1Δ control strain (FY1560). Each bar represents the average amount and standard error of three independent experiments.