3DA	DADADA motif
4PL	Four-parameter logistic
AF488	Alexa Fluor 488
AIDA-I	Adhesin Involved in Diffuse Adherence I
ARRIVE	Animal Research: Reporting of In Vivo Experiments
AT/ATs	Autotransporter(s)
AUC_ELISA	Area under the ELISA curve
AUC_FLOW	Area under the flow–cytometry curve
Bmax	Maximum binding (ELISA)
bn-mAbs	Broadly neutralizing monoclonal antibody
BSA	Bovine Serum Albumin
BSL-2	Biosafety Level 2
CDC	Centers for Disease Control and Prevention
CFU	Colony-Forming Units
CI/CIs	Confidence interval(s)
DBTL	Design–Build–Test–Learn
DTP	Diphtheria–Tetanus–Pertussis
EC50	Half-maximal effective concentration
ELISA	Enzyme-Linked Immunosorbent Assay
Env	Envelope glycoprotein (HIV)
FBS	Fetal Bovine Serum
FLIC	Flagellin fragment (TLR5 agonist)
FLOW	Flow–cytometry readout (context of manuscript)
FP	Fusion Peptide
FP1–FP4	Fusion Peptide variants 1–4
GDGDG	Acidic linker sequence
GGG	Glycine-repeat linker sequence
GRB	Genome-Reduced Bacteria
HBSS	Hank’s Balanced Salt Solution
HET3	Outbred HET3 mouse stock
HIV/HIV-1	Human Immunodeficiency Virus/type 1
HM	Homomeric (in vaccine names)
HRP	Horseradish Peroxidase
HT	Heteromeric (in vaccine names)
IBC	Institutional Biosafety Committee
ID50/ID80	50%/80% inhibitory dilution
IgG	Immunoglobulin G
IM	Intramuscular
Imm/Imms	Immunogen/Immunogens
L/L1/L2	Linkers (L1 = GDGDG; L2 = AQQASSS × 3)
LB	Lysogeny Broth (Luria–Bertani)
LMIC(s)	Low- and Middle-Income Countries
LPS	Lipopolysaccharide
mAb	Monoclonal antibody
mRNA	Messenger RNA
MPER	Membrane-Proximal External Region
NIAID	National Institute of Allergy and Infectious Diseases
OD450/OD600	Optical density at 450/600 nm
PCA	Principal Component Analysis
PC1	Principal Component 1
PBS	Phosphate-Buffered Saline
PEDV	Porcine Epidemic Diarrhea Virus
pLDDT	Predicted Local Distance Difference Test (score)
pTM	Predicted TM-score
RAU	Relative Antibody Units
RLU	Relative Light Units
rSIP	(Group B streptococcal) surface immunogenic protein
L3	Short flexible linker
SOC	Super Optimal broth with Catabolite repression
SOP(s)	Standard Operating Procedure(s)
TAE	Tris–Acetate–EDTA
TBST	Tris-Buffered Saline with Tween-20
Tfh	T follicular helper (cells)
TMB	3,3′,5,5′-Tetramethylbenzidine
TLR	Toll-Like Receptor
TZM-bl	Reporter cell line for HIV neutralization
UVA	University of Virginia
UV	Ultraviolet
UniProt	Universal Protein Resource (database)
KWC/GRB	Killed Whole-Cell genome-reduced Bacteria
KWC	Killed Whole-Cell
E. coli	Escherichia coli