Figure 2. Esketamine enhances the anti-proliferative effect of 5-FU in colon cancer cells. (A) Representative images (left) and quantification (right) of colony formation assays in DLD1 and HCT15 cells treated with esketamine (1 mM), 5-FU (30 µM), or their combination. Scale bars, 15 mm. (B) Representative images (left) and quantification (right) of EdU incorporation assays. Scale bars, 200 µm. For all panels, data are presented as the mean ± SD (n = 3 independent experiments). *p < 0.05, **p < 0.01, ****p < 0.0001.
