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. 2026 Jan 21;123(4):e2511733123. doi: 10.1073/pnas.2511733123

Fig. 2.

Nine-part figure shows N Q O 1 protein, densitometry, messenger R N A in P D A C PDXs, and clonogenic survival of A A 1444 P D X cells.

C29h is cytotoxic to PDAC cells with high NQO1 expression. (A) NQO1 protein in human cancer cell lines; ERK1/2, loading control. (B) Cleaved/total caspase-3 and PARP-1 in NQO1+ and NQO1− cancer lines treated for 48 h with the indicated C29h doses; actin, loading control; 0 = DMSO control. (C) C29h dose–response; IC50 from 72 h MTT assays. (D) Viability of parental and NQO1KO Capan-2 cells after 48 h treatment; data shown as % of DMSO controls (100%). Mean ± SD. Mean ± SD (n = 3 biological replicates). Two-way ANOVA, Dunnett’s multiple comparisons test; *P < 0.05, **P < 0.01, ****P < 0.0001, ns-not significant. (E–G) NQO1 protein (E), densitometry (F), and mRNA (G) in PDAC PDXs; MIA-PaCa-2 = 1.0 A.U.; actin, loading control. Mean ± SEM (n = 2 to 10 technical replicates). Kruskal–Wallis, Dunn’s multiple comparisons test; *P < 0.05, ***P < 0.001, ****P < 0.0001. (H) Clonogenic survival of AA1444 PDX cells ± 150 mM C29h for 8 d. (I) AA1444 viability after 48 h treatment; % MTT vs. NT control. One-way ANOVA, Dunnett’s multiple comparisons test; **P < 0.01, ***P < 0.001, ns-not significant.