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. 2026 Jan 21;123(4):e2511733123. doi: 10.1073/pnas.2511733123

Fig. 3.

A three-panel figure shows experimental schematics, I B of AA1444 P D A C cells, and viability of parental and N Q O 1 K O M I A-P a C a-2 cells.

Culture on ECM coated plates increases C29h cytotoxicity. (A) Experimental schematic (Top) and IB of AA1444 PDAC cells cultured on plastic (none) or ECM for 72 h (Bottom Left) with densitometric quantification (Bottom Right; A.U. vs. HSP90). HSP90 and ERK1/2, loading controls. Representative of two biological and three technical replicates. Mean ± SD (n = 6). Two-tailed unpaired t test; P < 0.05. (B) Experimental schematic and assessment of C29h and GEM cytotoxicity (SI Appendix, Fig. S3C). (C) Parental (CTRL) and NQO1KO MIA-PaCa-2 cells on plastic or ECM treated 48 h with 60 nM C29h. Viability (CCK-8) expressed as % of DMSO controls (100%). Mean ± SD (n = biological replicates). One-way ANOVA, Šídák’s test; P < 0.05, *P < 0.01, ***P < 0.0001, ns.