Skip to main content
. 2026 Jan 21;123(4):e2511733123. doi: 10.1073/pnas.2511733123

Fig. 6.

A multi-part figure shows the experimental design, tumor morphology, and m R N A and protein levels in C 57 B L/6n and NOD/S C I D mice.

C29h induced PDAC regression is lymphocyte dependent. (A) Experimental design and treatment scheme. (B) Gross morphology of tumor-bearing pancreata, treated as indicated (Left), and corresponding weights (Right). Mean ± SD (n = 3 to 8 mice), one-way ANOVA test, *P < 0.05; ***P < 0.001, ns-not significant. (C) H&E and IHC of pancreata with quantification. (Scale bars, 50 µm and 100 µm.) (H&E). Values from multiple fields were averaged per tumor; each point represents one tumor. Mean ± SD (n = 3 to 5 tumors). Kruskal–Wallis, Dunn’s multiple comparison test, *P < 0.05; **P < 0.01; ****P < 0.0001, ns-not significant. (D) Nqo1, Fap, and Cd44 mRNAs in pancreata from C57BL/6n and NOD/SCID mice under indicated treatments. Mean ± SD (n = 4 to 7). ANOVA, Holm–Šídák’s multiple comparisons test. *P < 0.05; ***P < 0.001, ns-not significant. (E) Cd8 mRNA (Top) and flow cytometry of immune cells from C57BL/6n pancreata (Bottom). CD45+ cells analyzed for CD8+, CD44+CD8+PD-1+ Tex, CD3e+CD4+Foxp3+ Treg, and CD11b+F4/80+PD-L1+ TAMs. Mean ±SEM (n/pancreata = 4 to 8, Top; 3 to 6, Bottom). One-way ANOVA, Holm–Šídák’s multiple comparisons test (Top), and multiple unpaired t tests (Bottom). *P < 0.05; ***P < 0.001; ****P < 0.000; ns-not significant.