Fig. 7.

Impact of CD4⁺ and CD8⁺ T cell depletion on C29h cytotoxicity. (A) Schematic of experimental design and treatments. Orthotopic pancreatic tumors were generated in C57BL/6 N mice and treated with C29h, CD4⁺ or CD8⁺ T cell–depleting antibodies, or isotype controls. (B) Gross morphology of tumor-bearing pancreata (Left) and corresponding tumor weights (Right). Each point = one tumor. Mean ± SD (n = 4 to 8 mice per group). One-way ANOVA with Šídák’s multiple comparisons test; *P < 0.05, **P < 0.01, ns-not significant. (C) Representative H&E and IHC for cCasp-3, CD8, and Ki-67 in pancreata from above-treated mice. (Scale bars, 50 µm.) (D) Quantification of IHC in the same pancreata; CD8⁺ T cell density normalized to tissue area (cells/mm²) and averaged per tumor. Data are shown as mean ± SEM (n = 4 to 8 mice per group). One-way ANOVA with Šídák’s multiple comparisons test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns-not significant.