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. 2025 Dec 27;16:3768. doi: 10.1038/s41598-025-33776-z

Table 6.

Spearman’s rank correlation analysis of African Penguin chosen spermatozoa parameters.

Viability1 Total motility Progressive motility Live2 HMMP
Viability1 1 0.41 0.26 0.8* 0.09
Total motility 0.56* 1 0.7* 0.33 0.69*
Progressive motility 0.33 0.8* 1 0.21 0.61*
Live2 0.6* 0.37 0.13 1 0.11
HMMP 0.23 0.44 0.43 0.23 1

Correlation coefficients calculated within the control group are presented in the upper triangle of the matrix, while those calculated within the T500 group are shown in the lower triangle. * correlation coefficients that were statistically significantly different from zero. The corresponding correlation coefficients were not statistically significantly different.

1 light microscopy assessment with eosin – nigrosine staining; 2 flow cytometry assessment with SYBR/PI staining; HMMP = High Mitochondrial Membrane Potential.