Distilled	Concentration
	Grams to	water	in 1X (after
Concentration	weigh	(ml)	dilution)
2.32 M NaCl	33.9	250	116 mM
108 mM KCl	2.0	250	5.4
16 mM MgSO4·7H2O	1.0	250	0.8
20 mM CaCl2·2H2O	0.75	250	1.0
0.50 M Trizma-HCl	19.75	250	25
4% Bovine serum albumin	10.0	250	0.2%
20 mM NaH2PO4	25 ml of	225	1.0
	0.2 M
40 mM Na2HPO4·7H20	50 ml of	200	2.0
	0.2 M